Hollies (Ilex L., Aquifoliaceae) includes more than 600 species worldwide and most of them have high economic and ornamental value. The young leaves of over 60 species are used in healthy beverages. Many holly species are widely grown in parks and gardens throughout the world for their foliage and decorative berries and hold an important position in gardens worldwide. During the past several years, genomic and genetic data have been rapidly accumulated for hollies. To store, inquire, mine, integrate and disseminate these large-scale datasets and to provide an omics data hub for the holly research and breeding programs, we launched a comprehensive holly database (Holly Genome & Taxonomy Database). Current Holly Genome Database integrated three detailed annotated genome references, re-sequencing data of over 110 holly species. In addition, a species list including species names, references, type information, and species phenotypic data were also provided. In order to enable users to access these data conveniently, three hierarchically accessible functions including Genome, Taxonomy, and Genotype were developed with easy-to-use browsers, search engines, etc. Furthermore, six built-in tools including Blast, JBrowse, Search Gene, Tissue expression, Gene Annotation, phylogenetic tree, Primer Design, and Literature were implemented or deployed for data downloading and browsing, gene functional exploration and experimental practice. Considering those together, we hope that the HollyGTB can effectively facilitate the breeding programs and scientific research for hollies.